By Prof. Humio Mizoguti MD, PhD (auth.)
Histological observations of human embryos hitherto were conducted utilizing paraffin sections of five to ten µm thickness, stained with the H-E approach or just with carmine. as a result of the thickness, cells are prepared in 2 or three layers in a single part and the histological information usually are not regularly transparent. This e-book offers particular morphological positive aspects of a truly good preserved human embryo with fifteen somites. The sections are approximately 0.75 µm thick and stained with toluidine blue. The thinness of sections and clearness of staining demonstrate the histological information of this embryo very competently. an entire set of top quality photomicrographs are offered for every of the chosen sections. The excessive answer of the photomicrographs will allow effortless comparability with the literature. The transparent presentation during this publication of embryonic improvement is more and more vital and hugely suitable for in-vitro fertilization, and therefore of curiosity to reproductive biologists in addition to anatomists.
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Extra info for A Fifteen-somite Human Embryo
The notochord is separated from both the ventral aspect of the rhombencephalon and the dorsal wall of the foregut. The myocardial tube of the ventriculus is still large, but the endocardial tube is no longer seen. The atrium elongates to the right, dorsal to the ventriculus, and its left and right halves each contain one endocardiac lumen. ' .. Pe ric ard iac cavity 39 Fig. 24. Section through posterior end of ventriculus (20). From the dorsolateral extremity of the rhombencephalon neural crest cells are migrating as small cell groups.
The lumen of the foregut markedly widens transversely, forming the first branchial pouch, which is separated from the first branchial groove by a two-cell-layer membrane, the branchial membrane. Dorsal and ventral to the foregut dorsal and ventral aortae are seen respectively on each side of the median line. The appearance of the bulbus cordis, ventriculus and pericardiac cavity is quite similar to that in Fig. 15 28 Tegmen rhombencephali Rhombocoele Neuroepithelium (rhombencephalon) Mesenchyme arch Aorta dorsalis 1st branchial pouch Foregut (pharynx) membrane groove Aortic sac Entoderm Aorta ventralis Endocardium Myocardium (bulb us cordis) Perlcardiac cavity Ectoderm · Pericard ium , • ,,: ' '.
Yolk sac 45 Fig. 27. Section through posterior portion of first somite and posterior end of foregut (32- 2). li but of a slightly thicker membrane, the roof plate. The entodermal epithelium lining of the ventral wall of the foregut elongates ventrally and connects with the entodermal lioing of the yolk sac. Lateral to the foregut is located the coelomic duct, and ventral to it the sinus venosus is embedded in the mesenchymal cells of the septum traosversum. -. Rhombocoele Neuroepithelium (rhombencephalon) Aorta dorsalis Entoderm Coelomic duct Foregut Amnion venosus Primordium of liver Yolk sac ....
A Fifteen-somite Human Embryo by Prof. Humio Mizoguti MD, PhD (auth.)