By L. Eggeling, H. Sahm, A. A. de Graaf (auth.), H. Sahm, C. Wandrey (eds.)
Contents:L. Eggeling, H. Sahm, A.A. de Graaf: Quantifying and Directing Metabolite Flux: program to Amino Acid Overproduction.-
R. Krämer: research and Modeling of Substrate Uptake and Product free up via Prokaryotic and Eukaryotic Cells.-
D. Weuster-Botz, A.A. de Graaf: response Engineering how you can learn Intracellular Metabolite Concentrations.-
W. Wiechert, A.A. de Graaf: In Vivo desk bound Flux research via 13C Labeling Experiments.-
B. Sonnleitner: New strategies for Quantitative Bioprocess learn and Development.
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7 References . . . . . . . . . . . . . . . . . . . . . . . . . 32 34 35 39 41 45 47 48 50 51 54 57 58 58 60 62 65 70 71 Translocation of molecules and ions across cell m e m b r a n e s is an important step for a complete description of the metabolic network in terms of kinetics, energetics and control. With a few exceptions, most molecules cross the permeability barrier of the m e m b r a n e with the aid of membrane-embedded carrier proteins.
2 Biochemistry of Transport Processes . . . . . . . . . . . . . . . . . 1 Transport Proteins and Mechanisms: Carriers, Channels, P u m p s . . . . . . . 2 Kinetics of Transport . . . . . . . . . . . . . . . . . . . . 3 Energetic Aspects of Carrier-Mediated Transport: The Concept of Coupling . . . . 4 Regulation of Transport Activity by the Cell . . . . . . . . . . . . . 3 Methodical Aspects .
8). This finding, together with the previously noted plasmid instabilities, indicated severe cellular consequences, and stimulated an assay for the intracellular metabolite concentrations. Extremely high concentrations close to 100 m m o l l - 1 threonine and homoserine are present, whereas in the parent strain the respective concentrations are below 10 m m o l l 1 (Fig. 8). This is certainly the reason for the instabilities observed. Most importantly, with three hom(FBR) copies threonine was accumulated intracellularly up to 100 m m o l l - 1 , but externally only 65 m m o l 1-1 was found.
Metabolic Engineering by L. Eggeling, H. Sahm, A. A. de Graaf (auth.), H. Sahm, C. Wandrey (eds.)